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Opened vial (after first puncture): The 28-day window begins the moment the rubber stopper is first punctured, regardless of how little volume is drawn
It is not intended for human consumption, veterinary use, therapeutic use, or clinical application
PMCID: PMC13026520
That means a thorough footwear audit replacing worn-out shoes, ditching completely flat footwear for anything involving significant walking, and modifying activities that load the injured tissue before it's ready
Phase I, n=42
After a B12 injection, avoid strenuous activity for 24 hours, refrain from soaking in hot baths or jacuzzis for at least 24 hours, and do not rub the injection site
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